Relative Effectiveness of Selenite Cystine Broth, Tetrathionate Broth, and Rappaport-Vassiliadis Medium for Recovery of Salmonella spp. from Raw Flesh, Highly Contaminated Foods, and Poultry Feed: Collaborative Study
Author:
June Geraldine A1, Sherrod Patricia S1, Hammack Thomas S1, Amaguaña R Miguel1, Andrews Wallace H1, Arling V, Ayers S, Ayotte E, Cirigliano M, Clifford D C, Cook D, Coles C, Dabney A, Davis T, Diaz B, Driggs R M, Eliasberg S, Fain A, Fung D Y C, Hammers A, Hu E, Jirele K, Keating K J, Kogan S, Kone K, Kuyyakamont B, Luebbert K, McDonagh S, McNally S, Mettler D, Milas J, Miller C, Nelson T, Nguyen P, Pfundheller R, Phebus R K, Redding R, Richardson S, Richter E, Robinson J, Romer J, Roo D W, Smoot L, Snow K, Tate C, Tompkins L, Vanderbilt K, Varney G W, Wagner D, Wang J, Wchienroj K,
Affiliation:
1. U.S. Food and Drug Administration, Division of Microbiological Studies, Washington, DC 20204
Abstract
Abstract
A collaborative study was performed in 18 laboratories to validate use of Rappaport-Vassiliadis (RV) medium in the standard culture method for recovery of Salmonella spp. from raw, highly contaminated foods and poultry feed. RV medium made from its individual ingredients and incubated at 42�C was compared with selenite cystine (SC) broth incubated at 35�C and tetrathionate (TT) broth incubated at 35� and 43�C for effectiveness in recovery of Salmonella spp. Four artificially contaminated foods (oysters, frog legs, mushrooms, and shrimp) and poultry feed and one naturally contaminated food (chicken) were analyzed. The artificially contaminated foods were inoculated with single serovars of Salmonella at target levels of 0.04 colony-forming units (CFU)/g for the low level and 0.4 CFU/g for the high level. For analysis of 1125 test portions, RV medium (42�C) recovered Salmonellairom 409 test portions; TT (43�C), from 368 test portions; TT (35�C), from 310 test portions; and SC (35�C), from 334 test portions. Overall, RV medium was comparable with or better than other selective enrichments for recovery of Salmonella from the foods in this study, except mushrooms. From mushrooms, SC broth (35�C) recovered more positive test portions than did RV medium (42�C) and TT broth (43�C). The method for detection of Salmonella in raw, highly contaminated foods and
Publisher
Oxford University Press (OUP)
Subject
Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry
Cited by
31 articles.
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