Negative supercoils regulate meiotic crossover patterns in budding yeast

Author:

Tan Taicong1,Tan Yingjin1,Wang Ying1,Yang Xiao12345,Zhai Binyuan12345,Zhang Shuxian16ORCID,Yang Xuan1,Nie Hui7,Gao Jinmin7ORCID,Zhou Jun7,Zhang Liangran167ORCID,Wang Shunxin12345

Affiliation:

1. Center for Reproductive Medicine, Cheeloo College of Medicine, State Key Laboratory of Microbial Technology, Shandong University , China

2. National Research Center for Assisted Reproductive Technology and Reproductive Genetics, Shandong University , Jinan, Shandong 250012, China

3. Key Laboratory of Reproductive Endocrinology of Ministry of Education , Jinan, Shandong 250001, China

4. Shandong Provincial Clinical Research Center for Reproductive Health , Jinan, Shandong 250012, China

5. Shandong Key Laboratory of Reproductive Medicine , Jinan, Shandong 250012, China

6. Advanced Medical Research Institute, Shandong University , Jinan, Shandong 250012, China

7. Center for Cell Structure and Function, Shandong Provincial Key Laboratory of Animal Resistance Biology, College of Life Sciences, Shandong Normal University , Jinan 250014, Shandong, China

Abstract

Abstract Interference exists ubiquitously in many biological processes. Crossover interference patterns meiotic crossovers, which are required for faithful chromosome segregation and evolutionary adaption. However, what the interference signal is and how it is generated and regulated is unknown. We show that yeast top2 alleles which cannot bind or cleave DNA accumulate a higher level of negative supercoils and show weaker interference. However, top2 alleles which cannot religate the cleaved DNA or release the religated DNA accumulate less negative supercoils and show stronger interference. Moreover, the level of negative supercoils is negatively correlated with crossover interference strength. Furthermore, negative supercoils preferentially enrich at crossover-associated Zip3 regions before the formation of meiotic DNA double-strand breaks, and regions with more negative supercoils tend to have more Zip3. Additionally, the strength of crossover interference and homeostasis change coordinately in mutants. These findings suggest that the accumulation and relief of negative supercoils pattern meiotic crossovers.

Funder

National Natural Science Foundation of China

Taishan Scholars Youth Project of Shandong Province

Publisher

Oxford University Press (OUP)

Subject

Genetics

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