Affiliation:
1. Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, People’s Republic of China
2. Beijing Elk Ecology Research Center, Daxing District, Beijing 100076, People’s Republic of China
Abstract
Abstract
A real-time qualitative and quantitative polymerase chain reaction method (cer-194) using the fluorescence dye EvaGreen and aimed at the cytochrome b sequence was established for detection of cervidae DNA in feedstuff. Eight meat meal samples derived from deer, bovine, ovine, camel, pig, rabbit, fish, and chicken and 17 cervidae hair samples covering 2 subfamilies, 4 genera, and 7 species were tested to prove the specificity of the cer-194 system and its universality within the cervidae family. Detection limit of 0.1 deer meat in fish meal, blood powder, and feather powder matrixes was confirmed.
Publisher
Oxford University Press (OUP)
Subject
Pharmacology,Agronomy and Crop Science,Environmental Chemistry,Food Science,Analytical Chemistry
Cited by
5 articles.
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