The role of lysophosphatidic acid acyltransferase 1 in reproductive growth of Arabidopsis thaliana

Author:

Barroga Niña Alyssa M1234,Nguyen Van C1ORCID,Nakamura Yuki12356ORCID

Affiliation:

1. RIKEN Center for Sustainable Resource Science (CSRS) , Yokohama 230-0045 , Japan

2. Institute of Plant and Microbial Biology, Academia Sinica , Taipei 11529 , Taiwan

3. Molecular and Biological Agricultural Sciences Program, Taiwan International Graduate Program, Academia Sinica and National Chung Hsing University , Taipei 11529 , Taiwan

4. Graduate Institute of Biotechnology, National Chung Hsing University , Taichung 40227 , Taiwan

5. Department of Biological Sciences, Graduate School of Science, The University of Tokyo , Tokyo 113-8654 , Japan

6. Biotechnology Center, National Chung Hsing University , Taichung 40227 , Taiwan

Abstract

Abstract Lysophosphatidic acid acyltransferase1 (LPAT1) catalyzes the second step of de novo glycerolipid biosynthesis in chloroplasts. However, the embryonic-lethal phenotype of the knockout mutant suggested an unknown role for LPAT1 in non-photosynthetic reproductive organs. Reciprocal genetic crossing of the lpat1-1 heterozygous line suggested a female gametophytic defect of the lpat1-1 knockout mutant. By suppressing LPAT1 specifically during seed development, we showed that LPAT1 suppression affected silique growth and seed production. Glycerolipid analysis of the LPAT1 knockdown lines revealed a pronounced decrease of phosphatidylcholine (PC) content in mature siliques along with an altered polyunsaturation level of the polar glycerolipids. In seeds, the acyl composition of triacylglycerol (TAG) was altered albeit not the content. These results indicate that plastidic LPAT1 plays an important role in reproductive growth and extraplastidic glycerolipid metabolism involving PC and TAG.

Funder

GteX Program Japan

Publisher

Oxford University Press (OUP)

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