STRUCTURAL ANALYSIS OF THE dur LOCI IN S. CEREVISIAE: TWO DOMAINS OF A SINGLE MULTIFUNCTIONAL GENE

Abstract

ABSTRACT In Saccharomyces cerevisiae, the degradation of urea to carbon dioxide and ammonia is catalyzed byurea carboxylase and albphanate hydrolase. The loci coding for these enzymes (dur1 and dur2) are very tightly linked an the right arm of chromosome II between pet11 and met8. Pleiotropic mutations that fail to complement mutations in either of the dur loci were found to be predominantly located in or near the dur2 locus. We interpret these data as suggesting that the two dur loci might in reality be domains of a single gene that codes fora multifunctional polypeptide. In view OIthis conclusion, we have renamed the dur loci as the durl,2 locus.