No Correlation Between Nasopharyngeal Human Bocavirus 1 Genome Load and mRNA Detection or Serology in Adeno-/Tonsillectomy Patients-Reference-Cited by-同舟云学术

No Correlation Between Nasopharyngeal Human Bocavirus 1 Genome Load and mRNA Detection or Serology in Adeno-/Tonsillectomy Patients

Published:2019-04-18 Issue:4 Volume:220 Page:589-593
ISSN:0022-1899
Container-title:The Journal of Infectious Diseases
language:en
Short-container-title:

Author:

Ivaska Lotta E¹^ORCID,Christensen Andreas²³,Waris Matti⁴,Puhakka Tuomo¹,Vuorinen Tytti⁴,Allander Tobias⁵,Söderlund-Venermo Maria⁶^ORCID,Jartti Tuomas⁷

Affiliation:

1. Department of Otorhinolaryngology – Head and Neck Surgery, Turku University Hospital and University of Turku, Finland

2. Department of Laboratory Medicine, Norwegian University of Science and Technology, Trondheim, Norway

3. Department of Clinical Microbiology, St Olavs Hospital, Trondheim University Hospital, Norway

4. Department of Virology and Clinical Virology, Turku University Hospital and University of Turku, Finland

5. Department of Clinical Microbiology, Karolinska University Hospital, Stockholm, Sweden

6. Department of Virology, University of Helsinki, Finland

7. Department of Pediatrics and Adolescent Medicine, Turku University Hospital and University of Turku, Finland

Abstract

Abstract Human bocavirus 1 (HBoV1) can persist in nasopharynx and tonsils. Using HBoV1 serology, reverse-transcription polymerase chain reaction (PCR) for detecting messenger RNA (mRNA) and quantitative PCR for HBoV1 genome load count, we studied to what extent the HBoV1 DNA loads in nasopharynx correlate with acute infection markers. Tonsillar tissue, nasopharyngeal aspirate, and serum were obtained from 188 elective adeno-/tonsillectomy patients. Relatively high loads of HBoV1 DNA were detected in the nasopharynx of 14 (7%) primarily asymptomatic subjects with negative mRNA and/or serodiagnostic results. Quantitative HBoV1 DNA PCR may have lower specificity than HBoV1 mRNA detection for diagnosing symptomatic infection.

Funder

Turku University Hospital Foundation

Turku University Foundation

Sigrid Jusélius Foundation

Life and Health Medical Association

Publisher

Oxford University Press (OUP)

Subject

Infectious Diseases,Immunology and Allergy

Link

http://academic.oup.com/jid/advance-article-pdf/doi/10.1093/infdis/jiz166/28667670/jiz166.pdf

Reference15 articles.