Affiliation:
1. Faculty of Life Sciences, Department of Bioscience, Tokyo University of Agriculture , 1-1-1 Sakuragaoka Setagaya-ku, Tokyo 1568502 , Japan
Abstract
Abstract
Our previous studies revealed that a two-component system (TCS), DevS, and DevR, regulate both nitric oxide (NO) signaling and NO homeostasis in the actinobacterium Streptomyces coelicolor A3(2) M145, suggesting a reasonable system for NO-dependent metabolism. In this study, sequence alignment of DevR and DevR homologs found Asp66 (D66) and Thr196 (T196) as predicted phosphorylation sites of DevR. Phos-tag gel electrophoretic mobility shift assay suggested that D66 and T196 are involved in the phosphorylation of DevR. The respective point mutations of D66 and T196 significantly decreased the transcriptional activity of DevR, which affected nitrite production and aerial mycelium formation. These results suggested that both D66 and T196 of DevR are important for the regulation of NO homeostasis and signaling in S. coelicolor A3(2) M145.
Funder
Japan Society for the Promotion of Science
Japan Science and Technology Agency
Tokyo University of Agriculture
Publisher
Oxford University Press (OUP)
Subject
Genetics,Molecular Biology,Microbiology