Modification of screen-printed gold electrode with 1,4-dithiothreitol: application to sensitive voltammetric determination of Sudan II

Abstract

Abstract Objectives The aim of this study is to investigate the electrochemical behavior of Sudan II (SuII) using a screen-printed gold electrode (SPGE) modified with 1,4-dithiothreitol (DTT) and to determine the amount of Sudan II by voltammetry. Materials and Methods A DTT-modified screen-printed gold electrode (DTT/SPGE) was fabricated and its application for differential pulse voltammetric (DPV) determination of SuII was reported. Fourier transform infrared spectroscopy (FT-IR), cyclic voltammetry and electrochemical impedance spectroscopy were used for the characterization of the modified electrode. The effects of instrumental and chemical parameters were optimized for the determination of SuII. The fabricated electrode was used for the analysis of SuII in fortified and real samples. High-performance liquid chromatography was preferred as a reference method for the evaluation of the obtained voltammetric results. Results The electrochemical studies and FT-IR demonstrated that the SPGE was modified with DTT. The obtained peak current at DTT/SPGE was 6.67 times higher than that recorded with SPGE. At the optimized conditions of DPV in pH = 2.5 of H2SO4, the oxidation peak current of SuII was proportional to its concentration in range: 0.001–1.500 μmol l–1 with a detection limit of 0.0002 μmol l–1 (S/N = 3). For the analysis of SuII, 101.67%–104.33% of recovery percentage was obtained. Conclusions A new electrode was successfully improved for the determination of SuII. This highly selective and sensitive electrode supplied the fast determination of SuII in ketchup, chili sauce and salsa dip sauce. In addition, voltammetric and chromatographic results are found to be consistent.