This chapter assesses the contribution of Regional Fisheries Management Organisations (RFMOs) to the achievement of the principles of conservation and cooperation articulated in the United Nations Convention on the Law of the Sea (LOSC). It begins with a brief historical introduction to the institutionalisation of cooperation through RFMOs and an examination of their structural limitations. It then considers the role and contribution of RFMOs in developing the specific content of the obligation to conserve, including the implications for RFMOs of the increasing recognition of the need to protect, conserve, and manage marine biodiversity in general. Finally, it examines the challenges to RFMOs posed by climate change.