Tyrosine phosphorylation on capacitated human sperm tail detected by immunofluorescence correlates strongly with sperm–zona pellucida (ZP) binding but not with the ZP-induced acrosome reaction
Author:
Publisher
Oxford University Press (OUP)
Subject
Obstetrics and Gynecology,Rehabilitation,Reproductive Medicine
Link
http://academic.oup.com/humrep/article-pdf/21/4/1002/1574168/dei435.pdf
Reference28 articles.
1. Asquith KL , Baleato RM, Mclaughlin EA, Nixon B and Aitken RJ (2004) Tyrosine phosphorylation activities surface chaperones facilitating sperm–zona recognition. J Cell Sci117,3645–3657.
2. Brewis IA , Clayton R, Barratt CLR, Hornby DP and Moore HDM (1996) Recombinant human zona pellucida glycoprotein 3 induces calcium influx and acrosome reaction in human spermatozoa. Mol Hum Reprod2,583–589.
3. Brewis IA , Clayton R, Browes CE, Martin M, Barratt CLR, Hornby DP and Moore HDM (1998) Tyrosine phosphorylation of a 95 kDa protein and induction of the acrosome reaction in human spermatozoa by recombinant human zona pellucida glycoprotein 3. Mol Hum Reprod4,1136–1144.
4. Buffone MG , Calamera JC, Verstraeten SV and Doncel GF (2005) Capacitation-associated protein tyrosine phosphorylation and membrane fluidity changes are impaired in the spermatozoa of asthenozoospermic patients. Reproduction129,697–705.
5. Cross NL , Morales P and Overstreet JW (1986) Two simple methods for detecting acrosome-reacted human sperm. Gamete Res5,213–226.
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