Evaluation of a Genetic Sexing Strain of the Oriental Fruit Fly as a Candidate for Simultaneous Application of Male Annihilation and Sterile Insect Techniques (Diptera: Tephritidae)

Abstract

Abstract The oriental fruit fly, Bactrocera dorsalis (Hendel), is an important pest of fruits and vegetables, and many countries operate surveillance programs to detect infestations and, if needed, implement costly control efforts. The Male Annihilation Technique (MAT), which involves deployment of the male lure methyl eugenol (ME) to reduce or eliminate the male population, has been used as a stand-alone strategy or as a precursor to the Sterile Insect Technique (SIT), which involves the release of sterile males to generate sterile male by wild female crosses and the production of inviable progeny. Modeling suggests that simultaneous implementation of MAT and SIT, rather than sequential deployment, increases the probability of successful eradication. Previous research has shown that B. dorsalis males that have fed on ME show reduced tendency to re-visit ME sources. Also, males fed ME gain a mating advantage over nonfed males. The goal of the present study was to evaluate the suitability of a genetic sexing strain of B. dorsalis as a candidate for concurrent implementation of MAT and SIT. Evaluation focused on the timing of prerelease exposure to methyl eugenol to identify the male age at which feeding upon the lure both i) reduces postrelease attraction to ME-baited traps (thus allowing the operation of MAT) and ii) enhances postrelease mating competitiveness (thus increasing the effectiveness of SIT). Results indicate that prerelease ME feeding by 6-d-old males, with release the following day, would allow effective, concurrent implementation of MAT and SIT.