Strong effects of lab-to-field environmental transitions on the bacterial intestinal microbiota of Mus musculus are modulated by Trichuris murisinfection

Author:

Bär Julian12ORCID,Leung Jacqueline M23ORCID,Hansen Christina2,Loke P'ng4,Hall Alex R1,Conour Laura5,Graham Andrea L2

Affiliation:

1. Department of Environmental Systems Science, ETH Zurich, 8092 Zurich, Switzerland

2. Department of Ecology and Evolutionary Biology, Princeton University, Princeton, NJ, 08544, USA

3. Department of Environmental Health Sciences, Columbia University, New York, NY, 10032, USA

4. Department of Microbiology, New York University Grossman School of Medicine, New York, NY, 10016, USA

5. Princeton Neuroscience Institute, Princeton University, Princeton, NJ, 08544, USA

Abstract

ABSTRACT Studies of controlled lab animals and natural populations represent two insightful extremes of microbiota research. We bridged these two approaches by transferring lab-bred female C57BL/6 mice from a conventional mouse facility to an acclimation room and then to an outdoor enclosure, to investigate how the gut microbiota changes with environment. Mice residing under constant conditions served as controls. Using 16S rRNA sequencing of fecal samples, we found that the shift in temperature and humidity, as well as exposure to a natural environment, increased microbiota diversity and altered community composition. Community composition in mice exposed to high temperatures and humidity diverged as much from the microbiota of mice housed outdoors as from the microbiota of control mice. Additionally, infection with the nematode Trichuris muris modulated how the microbiota responded to environmental transitions: The dynamics of several families were buffered by the nematodes, while invasion rates of two taxa acquired outdoors were magnified. These findings suggest that gut bacterial communities respond dynamically and simultaneously to changes within the host's body (e.g. the presence of nematodes) and to changes in the wider environment of the host.

Funder

Princeton University

National Science Foundation

ETH Zürich

Publisher

Oxford University Press (OUP)

Subject

Applied Microbiology and Biotechnology,Ecology,Microbiology

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