Generation of LexA enhancer-trap lines in <i>Drosophila</i> by an international scholastic network-Reference-Cited by-同舟云学术

Generation of LexA enhancer-trap lines in Drosophila by an international scholastic network

Published:2023-06-06 Issue:9 Volume:13 Page:
ISSN:2160-1836
Container-title:G3: Genes, Genomes, Genetics
language:en
Short-container-title:

Author:

Kim Ella S¹,Rajan Arjun²,Chang Kathleen²,Govindarajan Sanath¹,Gulick Clara¹,English Eva¹,Rodriguez Bianca³,Bloomfield Orion¹,Nakada Stella⁴,Beard Charlotte⁵,O’Connor Sarah⁶,Mastroianni Sophia⁶,Downey Emma⁶,Feigenbaum Matthew⁶,Tolentino Caitlin⁶,Pace Abigail⁶,Khan Marina⁶,Moon Soyoun⁶,DiPrima Jordan⁶,Syed Amber⁶,Lin Flora⁶,Abukhadra Yasmina¹,Bacon Isabella¹,Beckerle John¹,Cho Sophia¹,Donkor Nana Esi¹,Garberg Lucy¹,Harrington Ava¹,Hoang Mai¹,Lawani Nosa¹,Noori Ayush¹,Park Euwie¹,Parsons Ella¹,Oravitan Philip¹,Chen Matthew¹,Molina Cristian¹,Richmond Caleb¹,Reddi Adith¹,Huang Jason¹,Shugrue Cooper¹,Coviello Rose¹,Unver Selma¹,Indelicarto Matthew¹,Islamovic Emir¹,McIlroy Rosemary¹,Yang Alana¹,Hamad Mahdi¹,Griffin Elizabeth¹,Ahmed Zara¹,Alla Asha¹,Fitzgerald Patricia¹,Choi Audrey¹,Das Tanya¹,Cheng Yuchen¹,Yu Joshua¹,Roderiques Tabor¹,Lee Ethan¹,Liu Longchao¹,Harper Jaekeb¹,Wang Jason¹,Suhr Chris¹,Tan Max¹,Luque Jacqueline¹,Tam A Russell¹,Chen Emma¹,Triff Max¹,Zimmermann Lyric¹,Zhang Eric¹,Wood Jackie¹,Clark Kaitlin¹,Kpodonu Nat¹,Dey Antar¹,Ecker Alexander¹,Chuang Maximilian¹,López Ramón Kodi Suzuki¹,Sun Harry¹,Wei Zijing¹,Stone Henry¹,Chi Chia Yu Joy¹,Silvestri Aiden¹,Orloff Petra¹,Nedumaran Neha¹,Zou Aletheia¹,Ünver Leyla¹,Page Oscair¹,Kim Minseo¹,Chan Terence Yan Tao¹,Tulloch Akili¹,Hernandez Andrea¹,Pillai Aruli¹,Chen Caitlyn¹,Chowdhury Neil¹,Huang Lina¹,Mudide Anish¹,Paik Garrett¹,Wingate Alexandra¹,Quinn Lily⁵,Conybere Chris⁵,Baumgardt Luca Laiza⁵,Buckley Rollo⁵,Kolberg Zara⁵,Pattison Ruth⁵,Shazli Ashlyn Ahmad⁵,Ganske Pia⁵,Sfragara Luca⁵,Strub Annina⁵,Collier Barney⁵,Tamana Hari⁵,Ravindran Dylan⁵,Howden James⁵,Stewart Madeleine⁵,Shimizu Sakura⁵,Braniff Julia⁷,Fong Melanie⁷,Gutman Lucy⁷,Irvine Danny⁷,Malholtra Sahil⁷,Medina Jillian⁷,Park John⁷,Yin Alicia⁷,Abromavage Harrison⁷,Barrett Breanna⁷,Chen Jacqueline⁷,Cho Rachelle⁷,Dilatush Mac⁷,Gaw Gabriel⁷,Gu Caitlin⁷,Huang Jupiter⁷,Kilby Houston⁷,Markel Ethan⁷,McClure Katie⁷,Phillips William⁷,Polaski Benjamin⁷,Roselli Amelia⁷,Saint-Cyr Soleil⁷,Shin Ellie⁷,Tatum Kylan⁷,Tumpunyawat Tai⁷,Wetherill Lucia⁷,Ptaszynska Sara⁷,Zeleznik Maddie⁷,Pesendorfer Alexander⁷,Nolan Anna⁷,Tao Jeffrey⁷,Sammeta Divya⁷,Nicholson Laney⁷,Dinh Giao Vu⁷,Foltz Merrin⁷,Vo An⁷,Ross Maggie⁷,Tokarski Andrew⁷,Hariharan Samika⁷,Wang Elaine⁷,Baziuk Martha⁷,Tay Ashley⁷,Wong Yuk Hung Maximus⁷,Floyd Jax⁷,Cui Aileen⁷,Pierre Kieran⁷,Coppisetti Nikita⁷,Kutam Matthew⁷,Khurjekar Dhruv⁷,Gadzi Anthony⁷,Gubbay Ben⁷,Pedretti Sophia⁷,Belovich Sofiya⁷,Yeung Tiffany⁷,Fey Mercy⁷,Shaffer Layla⁷,Li Arthur⁷,Beritela Giancarlo⁷,Huyghue Kyle⁷,Foster Greg⁷,Durso-Finley Garrett⁷,Thierfelder Quinn⁷,Kiernan Holly⁷,Lenkowsky Andrew⁷,Thomas Tesia⁷,Cheng Nicole⁷,Chao Olivia⁸,L’Etoile-Goga Pia⁸,King Alexa⁸,McKinley Paris⁸,Read Nicole⁸,Milberg David⁸,Lin Leila⁸,Wong Melinda⁸,Gilman Io⁸,Brown Samantha⁸,Chen Lila⁸,Kosai Jordyn⁸,Verbinsky Mark⁸,Belshaw-Hood Alice⁸,Lee Honon⁸,Zhou Cathy⁸,Lobo Maya⁸,Tse Asia⁸,Tran Kyle⁸,Lewis Kira⁸,Sonawane Pratmesh⁸,Ngo Jonathan⁸,Zuzga Sophia⁸,Chow Lillian⁸,Huynh Vianne⁸,Yang Wenyi⁸,Lim Samantha⁸,Stites Brandon⁸,Chang Shannon⁸,Cruz-Balleza Raenalyn⁸,Pelta Michaela⁸,Kujawski Stella⁸,Yuan Christopher⁸,Standen-Bloom Elio⁸,Witt Oliver⁸,Anders Karina⁸,Duane Audrey⁸,Huynh Nancy⁸,Lester Benjamin⁸,Fung-Lee Samantha⁸,Fung Melanie⁸,Situ Mandy⁸,Canigiula Paolo⁸,Dijkgraaf Matijs⁸,Romero Wilbert⁸,Baula Samantha Karmela⁸,Wong Kimberly⁸,Xu Ivana⁸,Martinez Benjamin⁹,Nuygen Reena⁹,Norris Lucy⁹,Nijensohn Noah⁹,Altman Naomi⁹,Maajid Elise⁹,Burkhardt Olivia¹⁰,Chanda Jullian¹⁰,Doscher Catherine¹⁰,Gopal Alex¹⁰,Good Aaron¹⁰,Good Jonah¹⁰,Herrera Nate¹⁰,Lanting Lucas¹⁰,Liem Sophia¹⁰,Marks Anila¹⁰,McLaughlin Emma¹⁰,Lee Audrey¹⁰,Mohr Collin¹⁰,Patton Emma¹⁰,Pyarali Naima¹⁰,Oczon Claire¹⁰,Richards Daniel¹⁰,Good Nathan¹⁰,Goss Spencer¹⁰,Khan Adeeb¹⁰,Madonia Reagan¹⁰,Mitchell Vivian¹⁰,Sun Natasha¹⁰,Vranka Tarik¹⁰,Garcia Diogo³,Arroyo Frida³,Morales Eric³,Camey Steven³,Cano Giovanni³,Bernabe Angelica³,Arroyo Jennifer³,Lopez Yadira³,Gonzalez Emily³,Zumba Bryan³,Garcia Josue³,Vargas Esmeralda³,Trinidad Allen³,Candelaria Noel³,Valdez Vanessa³,Campuzano Faith³,Pereznegron Emily³,Medrano Jenifer³,Gutierrez Jonathan³,Gutierrez Evelyn³,Abrego Ericka Taboada³,Gutierrez Dayanara³,Ortiz Cristian³,Barnes Angelica¹¹,Arms Eleanor¹¹,Mitchell Leo⁴,Balanzá Ciara⁴,Bradford Jake¹²,Detroy Harrison¹²,Ferguson Devin¹²,Guillermo Ethel¹²,Manapragada Anusha¹²,Nanula Daniella¹²,Serna Brigitte¹²,Singh Khushi¹²,Sramaty Emily¹²,Wells Brian¹²,Wiggins Matthew¹²,Dowling Melissa⁹,Schmadeke Geraldine⁹,Cafferky Samantha¹⁰,Good Stephanie¹⁰,Reese Margaret¹⁰,Fleig Miranda¹⁰,Gannett Alex³,Cain Cory³,Lee Melody¹³,Oberto Paul¹⁴,Rinehart Jennifer¹⁴,Pan Elaine¹¹,Mathis Sallie Anne¹¹,Joiner Jessica⁴,Barr Leslie¹⁵,Evans Cory J¹²,Baena-Lopez Alberto¹⁶,Beatty Andrea⁶,Collette Jeanette⁶,Smullen Robert⁶,Suttie Jeanne⁶,Chisholm Townley¹,Rotondo Cheryl¹,Lewis Gareth⁵,Turner Victoria⁵,Stark Lloyd⁵,Fox Elizabeth⁷,Amirapu Anjana⁸,Park Sangbin²,Lantz Nicole⁷,Rankin Anne E¹,Kim Seung K²,Kockel Lutz²^ORCID

Affiliation:

1. Phillips Exeter Academy , Exeter, NH 03833 , USA

2. Department of Developmental Biology, Stanford University School of Medicine , Stanford, CA 94305 , USA

3. Pritzker College Prep , Chicago, IL 60639 , USA

4. Dalton School , New York, NY 10128 , USA

5. Haileybury School , Hertford SG13 7NU , UK

6. Commack High School , 1 Scholar Ln, Commack, NY 11725 , USA

7. The Lawrenceville School , 2500 Main St, Lawrenceville, NJ 08648 , USA

8. Lowell High School , 1101 Eucalyptus Dr, San Francisco, CA 94132 , USA

9. Latin School of Chicago , 59 W North Blvd, Chicago, IL 60610 , USA

10. Albuquerque Academy , Albuquerque, NM 87109 , USA

11. Chapin School , New York, NY 10028 , USA

12. Loyola Marymount University , Los Angeles, CA 90045 , USA

13. Harvard-Westlake School , Los Angeles, CA 90077 , USA

14. Hotchkiss School , Lakeville, CT 06039 , USA

15. Westtown School , West Chester, PA 19382 , USA

16. University of Oxford , Oxford OX1 3RE , UK

Abstract

Abstract Conditional gene regulation in Drosophila through binary expression systems like the LexA-LexAop system provides a superb tool for investigating gene and tissue function. To increase the availability of defined LexA enhancer trap insertions, we present molecular, genetic, and tissue expression studies of 301 novel Stan-X LexA enhancer traps derived from mobilization of the index SX4 line. This includes insertions into distinct loci on the X, II, and III chromosomes that were not previously associated with enhancer traps or targeted LexA constructs, an insertion into ptc, and seventeen insertions into natural transposons. A subset of enhancer traps was expressed in CNS neurons known to produce and secrete insulin, an essential regulator of growth, development, and metabolism. Fly lines described here were generated and characterized through studies by students and teachers in an international network of genetics classes at public, independent high schools, and universities serving a diversity of students, including those underrepresented in science. Thus, a unique partnership between secondary schools and university-based programs has produced and characterized novel resources in Drosophila, establishing instructional paradigms devoted to unscripted experimental science.

Funder

Stanford Vice Provost Undergraduate Education

Bio-X awards

John and Eileen Hessel Fund

Dayton Coles Science Research Fund

NIH awards

Stanford Diabetes Research Center

KM Mulberry Basic Science Endowed Professorship

Publisher

Oxford University Press (OUP)

Subject

Genetics (clinical),Genetics,Molecular Biology