Interplay between genetics and lifestyle on pain susceptibility in women with fibromyalgia: The al-Ándalus project

Author:

Estévez-López Fernando1,Guerrero-González Juan M2ORCID,Salazar-Tortosa Diego3,Camiletti-Moirón Daniel4,Gavilán-Carrera Blanca4,Aparicio Virginia A5,Acosta-Manzano Pedro6,Álvarez-Gallardo Inmaculada C4,Segura-Jiménez Víctor789,Soriano-Maldonado Alberto10,Geenen Rinie11,Delgado-Fernández Manuel6,Martínez-González Luis J2,Ruiz Jonatan R12,Álvarez-Cubero María J213

Affiliation:

1. Department of Child and Adolescent Psychiatry/Psychology, Erasmus MC, University Medical Center, The Netherlands, 3015 GD Rotterdam

2. GENYO (Pfizer-University of Granada-Andalusian Government Centre for Genomics and Oncological Research), Granada, Spain

3. Department of Ecology and Evolutionary Biology, University of Arizona, USA, Tucson, AZ 85719

4. Department of Physical Education, Faculty of Education Sciences, University of Cádiz, 11519 Cádiz, Spain

5. Department of Physiology, Faculty of Pharmacy, University of Granada, 18011 Granada, Spain

6. Department of Physical Education and Sport, Faculty of Sport Sciences, University of Granada, Spain, 18010 Granada

7. Instituto de Investigación Biosanitaria ibs. GRANADA, Spain, Granada

8. Hospital Universitario Virgen de las Nieves of Granada, Spain, Granada

9. GALENO research group, Department of Physical Education, Faculty of Education Sciences, University of Cádiz, Spain, Cádiz

10. Department of Education, Faculty of Education Sciences, University of Almería, Spain, 04120 Almería

11. Department of Psychology, Faculty of Social and Behavioural Sciences, Utrecht University, The Netherlands, 3508 TC Utrecht

12. PROFITH-”PROmoting FITness and Health Through Physical Activity”- Research Group, Department of Physical Education and Sport, Faculty of Sport Sciences, University of Granada, Spain, 18071 Granada

13. University of Granada, Department of Biochemistry and Molecular Biology III, Faculty of Medicine, Spain, PTS, Granada

Abstract

Abstract Objectives It is widely acknowledged that the experience of pain is promoted by both genetic susceptibility and environmental factors such as engaging in physical activity (PA) and that pain-related cognitions are also important. Thus, the purpose of the present study was to test the association of 64 polymorphisms (34 candidate-genes) and the gene-gene, gene-PA, and gene-sedentary behaviour interactions with pain and pain-related cognitions in women with fibromyalgia. Methods Saliva samples from 274 women with fibromyalgia (aged 51.7 ± 7.7 years) were collected for extracting DNA. We measured PA and sedentary behaviour by accelerometers for a week, pain with algometry and questionnaires, and pain-related cognitions with questionnaires. To assess the robustness of the results, a meta-analysis was also performed. Results The rs6311 and rs6313 polymorphisms (HTR2A) were individually related to algometer scores. The interaction of rs4818 (COMT) and rs1799971 (OPRM1) was related to pain catastrophizing. Five gene-behaviour interactions were significant: the interactions of sedentary behaviour with rs1383914 (ADR1A), rs6860 (CHMP1A), rs4680 (COMT), rs165599 (COMT) and rs12994338 (SCN9A) on bodily pain subscale of the SF-36. Furthermore, the meta-analysis showed an association between rs4680 (COMT) and severity of fibromyalgia symptoms (codominant model, p-value: 0.032). Conclusion The HTR2A gene (individually), COMT and OPRM1 gene-gene interaction, and the interactions of sedentary behaviour with ADRA1A, CHMP1A, COMT, and SCN9A genes were associated with pain-related outcomes. Collectively, findings from the present study indicate a modest contribution of genetics and gene-sedentary behaviour interaction to pain and pain catastrophizing in women with fibromyalgia. Future research should examine whether reducing sedentary behaviour is particularly beneficial for reducing pain in women with genetic susceptibility to pain.

Publisher

Oxford University Press (OUP)

Subject

Pharmacology (medical),Rheumatology

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