Current methods for monitoring Pseudomonas syringae biofilm development

Author:

Budil Jakub12,Lišková Petra23

Affiliation:

1. Institute of Physics of the Czech Academy of Sciences , Cukrovarnická 10, 162 00, Prague 6 , Czech Republic

2. Department of Genetics and Microbiology, Faculty of Science, Charles University , Viničná 5, 128 43, Prague 2 , Czech Republic

3. Research and breeding institute of pomology Holovousy Ltd. , Holovousy 129, 508 01, Horice , Czech Republic

Abstract

Abstract This work reviews biofilm investigation techniques and highlights the benefits and drawbacks of each approach focusing especially on Pseudomonas syringae and may serve as a comprehensive guide for any early-career researchers starting with the topic of biofilm. Each approach with applications of individual microscopy and spectroscopy techniques is summarized together with characterization of Pseudomonas syringae and its role in pathogenesis.

Funder

The Ministry of Education, Youth and Sports

Ministry of Agriculture of the Czech Republic

Publisher

Oxford University Press (OUP)

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