Inducing banana Fusarium wilt disease suppression through soil microbiome reshaping by pineapple–banana rotation combined with biofertilizer application

Abstract

Abstract. Crop rotation and biofertilizer application have historically been employed as efficient management strategies for soil-borne disease suppression through soil microbiome manipulation. However, how this occurs and to what extent the combination of methods affects the microbiota reconstruction of diseased soil is unknown. In this study, pineapple–banana rotation combined with biofertilizer application was used to suppress banana Fusarium wilt disease, and the effects on both bacterial and fungal communities were investigated using the MiSeq Illumina sequencing platform. Our results showed that pineapple–banana rotation significantly reduced Fusarium wilt disease incidence and the application of biofertilizer caused additional suppression. Bacterial and fungal communities thrived using rotation combined with biofertilizer application: taxonomic and phylogenetic α diversity of both bacteria and fungi increased along with disease suppression. Between the two strategies, biofertilizer application predominantly affected both bacterial and fungal community composition compared to rotation. Burkholderia genus may have been attributed to the general wilt suppression for its change in network structure and high relative importance in linear models. Our results indicated that pineapple–banana rotation combined with biofertilizer application has strong potential for the sustainable management of banana Fusarium wilt disease.