Spores of most common airborne fungi reveal no ice nucleation activity
Author:
Pummer B. G., Atanasova L., Bauer H., Bernardi J., Druzhinina I. S., Grothe H.ORCID
Abstract
Abstract. Fungal spores are ubiquitous biological aerosols, which are considered to show ice nucleation (IN) activity. In this study the respective IN activity was tested in oil emulsion in the immersion freezing mode. The focus was laid on species of economical, ecological or sanitary significance. For the first time, not only common moulds, but also edible mushrooms (Basidiomycota, Agaricomycetes) were investigated, as they contribute massively to the total amount of fungal spores in the atmosphere. Only Fusarium avenaceum showed freezing events at low subzero-temperatures, while the other investigated fungal spores showed no significant IN activity. Furthermore, we selected a set of fungal strains from different sites and exposed them to occasional freezing stress during cultivation. Although the total protein expression was altered by this treatment, it had no significant impact on the IN activity.
Publisher
Copernicus GmbH
Reference34 articles.
1. Ariya, P. A., Sun, J., Eltouny, N. A., Hudson, E. D., Hayes, C. T., and Kos, G.: Physical and chemical characterization of bioaerosols – implications for nucleation processes, Int. Rev. Phys. Chem., 28, 1–32, 2009. 2. Bauer, H., Kasper-Giebl, A., Löflund, M., Giebl, H., Hitzenberger, R., Zibuschka, F., and Puxbaum, H.: The contribution of bacteria and fungal spores to the organic carbon content of cloud water, precipitation and aerosols, Atmos. Res., 64, 109–119, 2002. 3. Bradford, M. M.: Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem., 72, 248–254, https://doi.org/10.1016/0003-2697(76)90527-3, 1976. 4. Frischmann, A., Neudl, S., Gaderer, R., Bonazza, K., Zach, S., Gruber, S., Spadiut, O., Friedbacher, G., Grothe, H., and Seidl-Seiboth, V.: Self-assembly at air/water interfaces and carbohydrate binding properties of the small secreted protein EPL1 from the fungus Trichoderma atroviride, J. Biol. Chem., 288, 4278–4287, 2013. 5. Fröhlich-Nowoiski, J., Pickersgill, D. A., Després, V. R., and Pöschl, U.: High diversity of fungi in air particulate matter, P. Natl. Acad. Sci. USA, 106, 12814–12819, 2009.
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献
|
|