Author:
Maulida Siti,Eriani Kartini,Fadli Nur,Handayani Luvi S.,Muchlisin Zainal A.
Abstract
Sperm cryopreservation is a sperm storage method that needs to be developed to ensure the availability of climbing perch fish (Anabas testudineus) sperm considering the difficulty of providing quality broodstock. This study aims to determine the effect of several types and concentrations of cryoprotectants on the DNA integrity of climbing perch fish sperm after cryopreservation for 14 days at a temperature of -196 ℃. Analysis of sperm DNA integrity used the gel electrophoresis method. This research consists of four types of cryoprotectants; Each type of cryoprotectant consists of two concentration levels. The cryoprotectant concentrations used were DMSO (concentrations 10 and 20%), Methanol (concentrations 10 and 20%), Glycerol (concentrations 5 and 20%), and Ethanol (concentrations 5 and 15%) as well as control treatment and fresh sperm treatment. Based on the results of the DNA integrity analysis of climbing perch fish sperm, it showed that all sperm DNA samples did not form fragments, meaning that there was no damage to climbing perch fish sperm DNA in all samples tested, both fresh sperm samples and post-cryopreserved sperm samples.