Performance of Mixed Cellulase-Amylase Immobilized on PSF Membrane Support via Cross-Linked with Glutaraldehyde in Enzymatic Hydrolysis

Abstract

A Cellulase and amylase are important enzymes for hydrolysis of cellulose and starchy material into the glucose. The performance of the mixed cellulose-amylase immobilized on polysulfone membrane (PSF) by cross-linked with glutaraldehyde was investigated. PSF membrane consists of 1-methyl-2-pyrolidone (NMP) as a solvent and using polyvinylpyrolidone (PVP) as an additive was developed. The study highlighted in the surface structure of PSF membrane, stability of the immobilized enzyme and reusability of the immobilized enzyme. Morphology studied using FESEM analysis indicated a good distribution of the pores was formed on the surface of the PSF membrane. The immobilization process shown no effect on the membrane structure and it was stable to be used as a support in immobilization process. The optimum operating condition for enzymatic hydrolysis of mixed cellulase-amylase on PSF membrane was 50°C and pH 5. The maximum glucose produced at the optimum condition was 4.843g/ml. The study also indicated that immobilized mixed cellulase-amylase achieved a maximum rate of reaction at first recycle of reusable before the rate of reaction decreased rapidly after 5 reusable hydrolysis cycles.