Determination of Sulfonamides in Synthetic Samples and Pharmaceutical Tablets Using HPLC-DAD Combined with SWATLD Algorithm

Abstract

The overlapping chromatograms and spectra of sulfonamides, sulfadiazine (SD) and sulfamethoxazole (SM) in both synthetic samples and pharmaceutical tablets in the presence of uncalibrated and interfering sulfanilamide (SN) were resolved by HPLC-DAD combined with SWATLD algorithm. The elution time was set from1.077 min to 1.624 min and the detection wavelength was chosen from 230 nm to 316 nm. The recoveries of SD and SM were (98.1 ± 4.8)% and (100.1 ± 1.6)%, respectively. The satisfying results show this method can be easily performed and applied to solving second-order calibration problems quickly and accurately.