Affiliation:
1. Wuhan Polytechnic University
2. Wuhan University of Technolgoy
Abstract
To detect DNA concentration, a novel fiber optic biosensor based on fluorescence quenching was designed and constructed by using lock-in amplifier for signal measurement. A linear relationship between the relative phase delay Δφof the sensing membrane and calf thymus DNA (ctDNA) concentration was observed in range from 2.0×10-9to 4.0×10-7M in the buffer of 5.0 mM Tris-HCl and 50.0 mM NaCl (pH 7.1), and the detection limit was 8.0×10-10M. The biosensor has a response time of 65 s and shows good stability and repeatability.
Publisher
Trans Tech Publications, Ltd.
Reference8 articles.
1. C.Z. Huang, K.A. Li, S.Y. Tong: Anal. Chem. Vol. 68(1996), p.2259.
2. J.R. Epstein, I. Biran, D.R. Walt: Anal. Chim. Acta Vol. 469(2002), p.3.
3. X. Wu, J.H. Yang, S. Sun, C.Y. Guo, D.H. Ran, J.H. Zheng: Luminescence Vol. 21(2006), p.129.
4. M.T. Li, J. Huang, X. Zhou, C. G Wang: Chin. J. Inorg. Chem. Vol. 24(2008), p.1794.
5. Y. Xiong, L.N. Ji: Coord. Chem. Rev. Vol. 185-186(1999), p.711.