Fast Determination of Active Components in Fructus Corni by Near Infrared Spectroscopy

Abstract

Today, near-infrared (NIR) has been proved to be a powerful analytical tool. It has been applied widely in agricultural, petrochemical, textile and pharmaceutical industries. In this paper, near-infrared spectroscopy (NIRS) combined with partical least square (PLS) was used as a qualitative tool to rapidly determinate two active components in Fructus Corni. The PLS calibration model of NIR Spectroscopy, the correlation coefficients (R2) of Loganin and Morroniside were 0.95895 and 0.98450, the root-mean-square error of cross-validation (RMSECV), the Correction of deviation, the prediction mean square error was 0.0344,0.109;0.0625, 0.2641 and 0.0948, 0.233. The result shows that, the near-infrared Reflectance Spectroscopy could be used to determinate the content of Loganin and Morroniside, and meanwhile as a simple and rapid new method for the quality assessment of Fructus Corni. In addition, the NIRS has a unique advantage in the quality control of traditional Chinese Medicine (TCM), such as rapid, accurate, nondestructive and no pollution. It is expected to be further uses in the quality control of TCM. It is can achieve the requirement of rapid detection of large quantities of Fructus Corni.