Purification of Enzymatically Produced Trehalose by Saccharomyces cerevisiae Strains Carrying Compound UV- and NTG-Induced Mutations

Author:

Piao Chun Hong1,Wang Yan1,Liu Jun Mei1,Yu Han Song1,Wang Yu Hua1

Affiliation:

1. Jilin Agricultural University

Abstract

Here, we describe a new method for the separation of trehalose after its enzymatic production. Compound mutagenesis of the Angel strain ofSaccharomycescerevisiaeusing ultraviolet irradiation and N-ethyl-N’-nitro-N-nitrosoguanidine resulted in three strains with high levels of maltose consumption, as determined by measurement of the residual maltose content of the culture medium. After 48 h of cultivation, the amount of residual maltose in the non-fermentation medium was 12.63%, a seven-fold reduction compared with that obtained with the original, non-mutated strain. Mutant strain G-4C3 produced trehalose with a purity of up to 96.69%.

Publisher

Trans Tech Publications, Ltd.

Subject

General Engineering

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3