Affiliation:
1. Universiti Teknologi Malaysia
2. Temasek Polytechnic
Abstract
In this study, PCR amplification of biodegradation related genes from NAR-2 bacterial consortium was accomplished. NAR-2 bacterial consortium consists of Citrobacter freundii A1, Enterococcus casseliflavus C1 and Enterobacter cloacae L17. The amplified genes were sequenced, bioinformatically analyzed and compared with the sequences from GenBank database of National Centre of Biotechnology Information (NCBI) using BLASTn and BLASTp search tools. The assembled sequences represented almost >70% of similarity to biodegradation related genes. These genes may act as a key intermediate enzyme in biodegradation pathway.
Publisher
Trans Tech Publications, Ltd.
Reference16 articles.
1. C. Rafols, D. Barcelo, Determination on mono- and disulphonated azo dyes by liquid chromatograpgy-atmospheric pressure ionization mass spectrometry, Journal of Chromatography A. 777 (1997) 177-192.
2. I.S. Thakur, Xenobiotics: pollutants and their degradation-mathane, benzene, pesticides, bioabsorption of metals, Environmental Microbiology.
3. A.A. Pandey, P. Singh, L. Iyengar, Bacterial decolorization and degradation of azo dyes, Journal of Biodeterioration and Biodegradation. 59 (2007) 73–84.
4. C.I. Pearce, J.R. Lloyd, J.T. Guthrie, The removal of color from textile waste water using whole bacterial cells: review, Dyes and Pigments. 58 (2003) 179-196.
5. G.F. Chan, N.A.A. Rashid, L.L. Koay, S.Y. Chang, W.L. Tan, Identification and optimization of novel NAR-1 bacterial consortium for the biodegradation of Orange II, Insight Biotechnol. 1 (2011) 7–16.