Cu Ion Release and Cell Damage Effect of Cu-Containing IUDs with Different Surface Areas of Copper

Abstract

The aim of the present study is to evaluate the cupric ion releases and cytotoxicity of Cu-bearing intrauterine device (IUD) with different surface areas. TCu380, GCu300 and MCu165 were selected and extracted with MEM for 24h. Inductively Coupled Plasma Mass Spectrometry (ICP-MS) was employed to determine the concentration of Cu ions. MTT assay was conducted to evaluate the cell viability of original and diluted extracts. Oxidative stress indicators such as superoxide dismutase (SOD) and lipid peroxidation (MDA) were measured. The results showed the concentration of Cu ions released from three kinds of IUDs were 114.836mg/L, 91.161mg/L and 85.750mg/L, respectively. Cell viability of all original IUDs extracts was around 10%. TCu380 showed more significant cytotoxicity and higher oxidative stress level than other two IUDs when extracts were diluted. The study suggested that there was a positive correlation between the quantity of released Cu ions and cell damage effect in Cu-IUDs. The mechanism of cytotoxicity might be elevation of oxidative stress.