Polygoni Cuspidati Radix Inhibits the Activation of Syk Kinase in Mast Cells for Antiallergic Activity

Author:

Lim Beong Ou1,Lee Jun Ho1,Ko Na Young1,Mun Se Hwan1,Kim Jie Wan1,Kim Do Kyun1,Kim Ju Dong1,Kim Bo Kyung1,Kim Hyung Sik1,Her Erk1,Lee Hoi Young1,Choi Wahn Soo1

Affiliation:

1. College of Biomedical & Health Science, Konkuk University, Chungju 380-701, Korea; Department of Immunology and Physiology, College of Medicine, Konkuk University, Chungju 380-701, Korea; College of Pharmacy, Pusan National University, Busan 609-735, Korea; and College of Medicine, Konyang University, Nonsan 320-711, Korea

Abstract

The antiallergic activity of Polygoni cuspidati radix (PR) and the mechanism of action by which it functions were investigated in this study. The extract of PR exhibited potent inhibitory activity in mast cells; its IC50 values were 62 ± 2.1 μg/ml for RBL-2H3 mast cells and 46 ± 3.2 μg/m for bone marrow–derived mast cells by antigen stimulation, and it also suppressed the expression of tumor necrosis factor-α and interleukin-4 in RBL-2H3 cells. According to the in vivo animal allergy model, it inhibited a local allergic reaction, passive cutaneous anaphylaxis, in a dose-dependent manner. With regard to its mechanism of action, PR inhibited the activating phosphorylation of Syk, a key signaling protein for the activation of mast cells. It also suppressed Akt and the mitogen-activated protein kinases ERK1/2, p38, and JNK, which are critical for the production of various inflammatory cytokines in mast cells. The results of the study indicate that the antiallergic activity of PR is mediated through the inhibition of histamine release and allergic cytokine production by the inhibition of Syk activating phosphorylation in mast cells.

Publisher

SAGE Publications

Subject

General Biochemistry, Genetics and Molecular Biology

Reference33 articles.

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3