The Impact of Nanomaters on the Microbial Infection on a Wooden Coffin Covered with a Layer of Black Resin and Coloured Materials

Abstract

The goal of this work is to use novel mixed additives of Nano Chitosan combined with nanoparticles and other substances that could inhibit the microbial growth of the wooden coffin. The wooden coffin, covered with a layer of black resin and coloured materials, was found in bad condition, and covered with a thick layer of dust. Several ties were used to preserve the sides of the coffin in the previous restoration. Cellulose agar was used to cultivate fungi, and nutrient agar was used to cultivate bacteria. The fungi were Aspergillus flavus, Cladosporium herbarum, and Aspergillus niger, while the bacteria were G+ve Bacillus sp., Bacillus megatrium, and Bacillus jeotgali. composite of ZnO NP, Ag NP, p-chloro-m-crysol (PCMC), Shim plant (Sh) with Nano Chitosan with 1, 2, and 3% concentrations were chosen for the purpose of treating microbial infections and determining which materials would work best to prevent microbial growth. Nano-chitosan + Ag NP and ZnO NP (1%) gave the best inhibition for Aspergillus niger (30mm). Nano-chitosan + PCMC (1%) gave the best inhibition for Aspergillus flavus (25mm), Cladosporium herbarum (25mm), and Bacillus jeotgali (40mm). The same effect was found for Nano-chitosan + PCMC (1%) and Nano-chitosan for Bacillus megatrium and G+ve Bacillus sp. (40mm).