SYNTHESIS, CHARACTERIZATION AND IN VITRO EVALUATION OF VATERITE MICROPARTICLES

Abstract

Objective: The aim of this research is to synthesize submicron-sized calcium carbonate vaterite particles of elliptical shape with different reaction durations. We also aim to assess their antioxidant and anti-inflammatory properties, which may be beneficial for treating diseases such as asthma and rheumatoid arthritis. Methods: Calcium carbonate vaterite particles were prepared using the co-precipitation method with varying reaction times, characterized by Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Fourier Transformation Infrared Spectroscopy (FTIR), and Poly-Dispersity Index, with antioxidant activity assessed by the 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) method and anti-inflammatory activity by the protein denaturation method. Results: SEM and TEM analysis revealed that the synthesized vaterite particles had an elliptical shape with nano-crystalline particles of around 50 nm size. FTIR verified the production of vaterite particles. Research on antioxidants and anti-inflammatory agents revealed that the crystalline particles exhibited DPPH scavenging action, with an IC50 of 12.6 µg/ml, and a noteworthy reduction in protein denaturation in the albumin protein denaturation test, with an IC50 of 222.49 µg/ml, in comparison to the reference value. Conclusion: The results highlight the potential of elliptical vaterite submicron micro-particles as versatile platforms with anti-inflammatory and antioxidant properties, paving the way for future advancements in drug delivery systems, food additives, and natural supplements by efficiently encapsulating drugs and proteins.