EXTRACTION OF FLAVONOID FROM VARIOUS PARTS OF COUROUPITAGUIANENSIS AND ITS EFFICACY AGAINST PATHOGENIC BACTERIA

Abstract

Objective: Quantitative, qualitative, high performance liquid chromatography (HPLC) analysis of flavonoids content from different parts of Couroupita guianensis and antibacterial activity against various pathogenic bacteria.Methods: Different parts of C. guianensis such as leaves, stem bark, flower anther, flower petals, fruit rind, and fruit pulp were collected and used for extraction  of active constituents. The phytochemical analysis was conducted to identify the presence of flavonoids in different plant parts. The amount of flavonoids were analyzed by quantitative analysis. Furthermore, HPLC analysis was performed to isolate and identify the flavonoids from the different parts. The methanol extract of different plant parts was also used to test the antibacterial efficacy in different human bacterial pathogens.Results: Flavonoids demonstrate a wide range of biochemical and pharmacological effects. In this study, we identified the flavonoids content in different parts of C. guianensis. The HPLC analysis has significantly proved the presence of flavonoids in different plants parts and amount of flavonoids differs in different parts of the plant. The results also showed the significant efficacy of its extract to the different pathogenic bacterial strains.Conclusion: Our study suggested that C. guianensis is a richer source of flavonoids and due to a higher amount of flavonoids; it may have enormous potential to scavenge the free radicals, oxidative damage of cell during different stresses including bacterial infections. We also suggested that HPLC analysis is efficient method to isolate and identify the different compounds and study can further extend to identify and isolate the different novel compounds from C. guianensis.Keywords: Couroupita guianensis, High performance liquid chromatography, Flavonoids, Antibacterial activity.