Affiliation:
1. Laboratory of Genetics, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892
2. Laboratoire d'Immunohematologie et Immunopathologie, Institut Pasteur, 75724 Paris Cedex 15, France
Abstract
The many binding studies of monoclonal immunoglobulin (Ig) produced by plasmacytomas have found no universally common binding properties, but instead, groups of plasmacytomas with specific antigen-binding activities to haptens such as phosphorylcholine, dextrans, fructofuranans, or dinitrophenyl. Subsequently, it was found that plasmacytomas with similar binding chain specificities not only expressed the same idiotype, but rearranged the same light (VL) and heavy (VH) variable region genes to express a characteristic monoclonal antibody. In this study, we have examined by enzyme-linked immunosorbent assay five antibodies secreted by silicone-induced mouse plasmacytomas using a broader panel of antigens including actin, myosin, tubulin, single-stranded DNA, and double-stranded DNA. We have determined the Ig heavy and light chain V gene usage in these same plasmacytomas at the DNA and RNA level. Our studies reveal: (a) antibodies secreted by plasmacytomas bind to different antigens in a manner similar to that observed for natural autoantibodies; (b) the expressed Ig heavy genes are restricted in V gene usage to the VH-J558 family; and (c) secondary rearrangements occur at the light chain level with at least three plasmacytomas expressing both κ and λ light chain genes. These results suggest that plasmacytomas use a restricted population of B cells that may still be undergoing rearrangement, thereby bypassing the allelic exclusion normally associated with expression of antibody genes.
Publisher
Rockefeller University Press
Subject
Immunology,Immunology and Allergy
Reference67 articles.
1. Plasmacytomagenesis in micemodel of neoplastic development dependent upon chromosomal translocations;Potter;Carcinogenesis.,1992
2. Antigen-binding myeloma proteins of mice;Potter;Adv. Immunol.,1977
3. Naturally occurring antibodies against nine common antigens in human sera. I. Detection, isolation and characterization;Guilbert;J. Immunol.,1982
4. Murine hybridomas secreting natural monoclonal antibodies reacting with self antigens;Dighiero;J. Immunol.,1983
5. Structural and affinity studies of IgM polyreactive natural autoantibodies;Diaw;J. Immunol.,1997
Cited by
19 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献