INOSITOLS: BIOLOGICAL ROLE AND APPLICATION, METHODS OF EXTRACTION FROM PLANT RAW MATERIALS AND DETERMINATION, BIOTECHNOLOGICAL SYNTHESIS

Abstract

The aim of the work was to review modern extraction, detection and quantification analytical methods of inositols and their derivatives. Methods. Inositols are extracted from vegetable raw materials by methods of liquid extraction, under pressure, microwave extraction and supercritical fluid extraction. Quantitatively analyzed by methods of gas and liquid chromatography with preliminary derivatization. The structure of inositols can be determined by the NMR spectroscopy. Results. Inositols and their derivatives are biologically active compounds, wich are involved in the egulation of the intracellular calcium level, the transmission of hormonal signals, the breakdown of fats and the reduction of cholesterol in the blood, the modulation of the neurotransmitters activity, etc. Inositols are used in the production of vitamin preparations. The main source for inositols extraction is vegetable raw material, namely alfalfa, as well as wheat sprouts, grapefruit, hazelnuts and others. In the paper, the methods of inositols extraction with organic and inorganic solvents, including the use of a Soxhlet apparatus, liquid extraction under pressure, microwave extraction and supercritical fluid extraction are considered. The procedure of preliminary sample preparation and polyols derivatization for their further separation and quantitative determination is described. Modern chromatographic methods of polyols identification and quantitative determination are analyzed. The possibility of using 1H, 13C and 31P NMR spectroscopy to identify the structure of inositols and their derivatives is described. Conclusions. Inositols are biologically active compounds of a wide spectrum of action, therefore there is an urgent need to develop biotechnological processes for their production and extraction from plant raw materials and microorganisms.