Affiliation:
1. Department of Pharmaceutical Quality Assurance, B.K. Mody Government Pharmacy College, Polytechnic Campus, Near Aji Dam, Rajkot-360003, India
Abstract
RP-UPLC method was developed and validated for the determination of chlorpheniramine maleate
and dextromethorphan hydrobromide in tablet dosage form. Reverse phase waters acquity UPLC
BEH C18 (50 mm × 2.1 mm, 1.7 μm) column using isocratic mobile phase of 0.5 mL 0.1% TFA
(trifluroacetic acid) in H2O:CH3CN (70:30 %v/v). The flow rate was 0.2 mL/min and 252 nm wavelength
use for detection on PDA detector. The retention time of chlorpheniramine maleate was 1.2 min and 2.2
min for dextromethorphan hydrobromide. Chlorpheniramine maleate and dextromethorphan
hydrobromide was subjected to stress conditions including acidic, alkaline, oxidation, photolysis and
thermal degradation. The method was validated as per ICH guideline with respect to samples to
specificity, precision, accuracy, linearity and robustness.
Publisher
Asian Journal of Chemistry