Affiliation:
1. Department of Chemistry, S.S. Margol Degree College, Shahabad-585228, India
Abstract
Quantitative spectrophotometric determination of cefpirome in pure and pharmaceutical dosage has
been developed. Method I produces a pink-coloured chromogen peak at λmax 510 nm by reacting
diazotized cefpirome drugs with diphenylamine (DPA) in a neutral medium. Method II obtained of a
coloured Schiff bases when cefpirome reacts with alcoholic p-dimethylaminobenzaldehyde (PDAB)
to produce a yellow-coloured chromogen with a maximum absorption wavelength of 415 nm. In both
methods I and II, Beer’s law is followed in the concentration ranges of 0.3-3.0 and 0.5-5.0 μg/mL,
respectively, with molar absorptivity of 5.13 × 104 and 2.54 × 104 for each form. At three separate
concentrations, intra-day and inter-day (RSD) and relative error (RE) are measured. The current methods
are simple, reliable, inexpensive, speedy and highly reproducible and have been tested in broad range
of pharmaceutical formulations with statistical comparisons to reference methods.
Publisher
Asian Journal of Chemistry