Effects of melatonin on growth and antioxidant capacity of naked oat (Avena nuda L) seedlings under lead stress

Abstract

Melatonin (MT) plays an important role in plant response to abiotic stress. In recent years, lead (Pb) pollution has seriously affected the living environment of plants. In this study, we applied two different concentrations of MT to naked oat seedlings under Pb stress to explore the effect of MT on naked oat seedlings under Pb pollution. The results showed that Pb stress seriously inhibited the growth and development of naked oat seedlings, which was alleviated by MT. MT could increase the soluble protein content and decrease the proline content of naked oat seedlings to maintain the osmotic balance of naked oat seedlings. The application of MT could accelerate the removal of reactive oxygen species (ROS) and improve the activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), so as to maintain the redox balance in naked oat seedlings. Exogenous melatonin could significantly increase the chlorophyll content of naked oat seedlings under Pb treatment, so as to improve the photosynthesis efficiency of naked oat seedlings. MT could also remarkably up regulate the expression of the genes of LOX, POX and Asmap1, and affect the expression of transcription factors NAC and WRKY1. It might regulate the expression of downstream genes through MAPKs pathways and TFs to improve the Pb tolerance of naked oat seedlings. These results proved that MT could significantly promote the growth and development of naked oats seedlings under Pb stress, which is expected to be applied in agricultural production practice.