Affiliation:
1. Department of Human Anatomy, Redeemer’s University, Ede, Osun State, Nigeria
2. Department of Anatomy, University of Benin, Benin City, Edo State, Nigeria
Abstract
Introduction: Generally, factors that up-regulate gastric mucosal protective factors or down-regulate aggressive factors contribute to the maintenance of mucosal integrity. This study was done to assess the role of mucous cells, Bcl-2 and p53 proteins during the gastroprotective activity of aqueous extract of Ageratum conyzoides.Methods: The phytochemistry of A. conyzoides extract was analyzed using a gas chromatography-mass spectrometer. Animals were subdivided into five groups, including non-treated normal control group A, non-treated test control group B, and treated groups C-E (Pre-treated with 100, 300, and 500 mg/kg A. conyzoides, respectively for 28 days). After the treatment period, pyloric-ligation was used to induce mucosal injury. Gastric tissues were harvested, grossly examined, and processed for histological, histochemical, and immunohistochemical studies. Stained sections were examined and quantified using image-J software. The data were analyzed using IBM-SPSS (version 23), and comparisons were checked via t test and analysis of variance.Results: Mild mucosal erosion was observed in the treated groups, but intense erosion was prominent in the test control animals. There was an insignificant increase in mucous cells, a significant (P < 0.05) increase in Bcl-2 expression without a significant increase in p53 expression in gastric mucosa of pre-treated animals compared to normal control. Gastric mucosa of test control showed a significant (P < 0.05) decrease in mucous cell count and Bcl-2 expression with a significant concomitant increase in p53 expression.Conclusion: Increased mucous cell population and reciprocal expressions of Bcl-2 and p53 proteins in the gastric mucosa of animals highlighted the sub-cellular mechanisms of gastroprotective activity of A. conyzoides.
Publisher
Maad Rayan Publishing Company
Reference35 articles.
1. Omotoso DR, Uwagbor V, Lawal OS, Olatomide OD, Okojie IGGross and histo-morphological study of anti-ulcerogenic effects of Cissampelos owariensis (PBeauv) methanolic extract in Wistar rats;Omotoso DR;J Biomed Sci,2019
2. Sembulingam K, Sembulingam P. Essentials of Medical Physiology. 5th ed. St. Louis, USA: Jaypee Brothers Medical Publishers Ltd; 2010. p. 218-26.
3. Rhoades RA, Tanner GA. Medical Physiology. 2nd ed. Lippincott Williams & Wilkins; 2004. p. 484-88.
4. Johnson LR. Essential Medical Physiology. 3rd ed. Carlifonia, USA: Academic Press; 2003. p. 502-12.
5. Induction of cyclooxygenase-2 in rat gastric mucosa by rebamipide, a mucoprotective agent;Sun WH;J Pharmacol Exp Ther,2000