Levels of retinyl palmitate and retinol in stratum corneum, epidermis and dermis of SKH-1 mice

Author:

Yan Jian1,Xia Qingsu1,Webb Peggy1,Warbritton Alan R2,Wamer Wayne G3,Howard Paul C1,Boudreau Mary1,Fu Peter P4

Affiliation:

1. National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079, USA

2. Toxicological Pathology Associates, Jefferson, AR 72079, USA

3. Center for Food Safety and Applied Nutrition, US Food and Drug Administration, College Park, MD 20740, USA

4. National Center for Toxicological Research, US Food and Drug Administration, Jefferson, AR 72079, USA,

Abstract

Vitamin A (retinol) regulates many biological functions, including epidermal cell growth. Retinyl palmitate (RP) is the major esterified form of retinol and the predominant component of retinoids in the skin; however, how endogenous levels of RP and retinol in the skin are affected by the age of the animal remains unknown. Furthermore, the levels of retinol and RP in the various skin layers- the stratum corneum, epidermis and dermis of skin- have not been reported. In this paper, we report the development of a convenient method for separation of the skin from SKH-1 female mice into the stratum corneum, epidermis, and dermis and the determination of the levels of RP and retinol in the three fractions by HPLC analysis. The total quantities of RP and retinol from the stratum corneum, epidermis, and dermis are comparable to those extracted from the same amount of intact skin from the same mouse. There was an age-related effect on the levels of RP and retinol in the skin and liver of female mice. An age-related effect was also observed in the stratum corneum, epidermis, and dermis. The levels of RP and retinol were highest in the epidermis of 20-week-old mice, and decreased when the age increased to 60- and 68-weeks. The total amount of RP at 20 weeks of age was found to be 1.52 ng/mg skin, and decreased about 4-fold at 60- and 68-weeks of age. A similar trend was found for the effects of age on the levels of retinol.

Publisher

SAGE Publications

Subject

Health, Toxicology and Mutagenesis,Public Health, Environmental and Occupational Health,Toxicology

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