Abstract
Objectives: This study aims to provide a feasible method for culturing tumor stem cells to facilitate further research on the occurrence and development of brain tumors, making it applicable for in vitro studies of brain tumor stem cells. Methods: Samples of glioma were collected and subsequently isolated and cultured, then immunocytochemical staining, including CD133, Nestin, and GFAP staining, was performed on the cultured cells. Results: Some of the primary cultured tumor stem cells grew in the shape of sphere and formed into clusters. With the extension of culture time, the cell mass gradually increased, with strong proliferation and self-renewal ability. Meanwhile, the tumor derived spherical cells could differentiate into different types of tumor cells in serum-containing medium since most of them were positive to CD133, Nestin, and GFAP on the 7 days. Conclusion: The tumor stem cell can differentiate into different cell types, carrying significant implications for tumor research and treatment. More importantly, the methods introduced in this study are simple and feasible to isolate and culture tumor stem cell, contributing to cell source for future studies.