Plasma Activated Water Solution as an Efficient Approach for Antiseptic and Wound Healing Applications: An Animal Model

Abstract

The current investigation was aimed at identifying the potential of plasma activated water as an antiseptic and wound-healing solution. The simplicity and affordability of the indirect approach of plasma therapies led us to evaluate rinsing of wounds via plasma activated water (PAW) and compare to the direct approach of plasma jet. Although previous studies have shown the effectiveness of plasma-activated water in wound healing and disinfection, this study was conducted to investigate two PAWs with various compositions in wound management. In this regard, PAWs with two different treatment times were produced and used for rinsing of wounds. H₂O₂ and NO₂- concentrations as well as acidity values of PAW were measured. Wound healing was investigated to find an efficient solution using Masson trichrome and hematoxylin and eosin staining. The antibacterial effect and wound closure rate of the directly applied plasma jet were significantly improved compared to the PAW treatments. The results exhibited an increase in collagen expression, resulting in the best rate of wound closure. Thus, our results suggested that plasma jet treatment and PAW rinsing of wounds are effective methods without side effects in promoting wound healing.