effect of MgO NPs that synthesis by Streptococcus spp. on immune system in rabbits

Abstract

Abstract The current study describes a low-cost, environmentally friendly, and repeatable magnesium oxide nanoparticle manufacturing process mediated by Streptococcus spp.  The UV spectrophotometer, Atomic Force Microscopy (AFM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and Field Emission Scanning Electron Microscopy (FE-SEM) were all used to characterize the nanoparticles. The crystallographic cubic pattern seen by the XRD indicates that the structure of nanoparticles is mostly crystalline. The characterisation peaks and functional groups of MgO nanoparticles were identified using FTIR analysis. Furthermore, the study of MgO NPS produced by S. salivarius and S. mutans using field-emission scanning electron microscopy (FESEM) revealed that their average diameters were 49.85 nm and 66.15 nm, respectively. The findings of all immunological tests conducted in this investigation showed that MgO NPs produced by S. mutans and S. salivarius were substantially different from the control group (P 0.05) for systemic and mucosal antibody, respectively. In comparison to the control group, which had a mean value of (10)(1), MgO NPs biosynthesized from S. mutans and S. salivarius generated titers with mean values (1280)(2560) and (128)(256), respectively. In contrast to the control group, where the mitotic index resulted in a mean value of (3.56), the findings for the mitotic index were (10.16)(7.83). LIF in the test groups (51.33)(44.1) were significant compared to the control group (102.5).The MgO NPs elicited cellular immunity (delayed type hypersensitivity), which led to an increase in the spleen weight index in both test groups compared to the control.