A Preliminary Study on Oxalate Degradation by <i>Lactobacillus</i> Isolated from canine’s faeces

Abstract

This is a preliminary study that reveals the importance of utilizing the potential microorganism for efficient oxalate degradation in the human gut. Hyperoxaluria is considered as the major risk factors related to recurrent ureolithiasis and progressive nephro-calcinosis. The organism isolated was confirmed to strain level by 16SrRNA sequencing and BLAST analysis.<i> Lactobacillus plantarum</i>, a gram positive bacterium isolated from the dog faeces was found to show a wide range of oxalate degradation in vitro, especially with sodium and ammonium oxalate. To check the viability of <i>Lactobacillus plantarum</i> in gastro- intestine, acid tolerance test was done to confirm whether the organism can survive the gastrointestinal pH of about 1-2.5. <i>Lactobacillus plantarum</i> is acid tolerant as well as bile tolerant as it can grow in the pH range of 3-7 and 0.1-3% bile salts. These properties make< i>Lactobacillus plantarum</i> a beneficial organism thereby opening a new platform to be used as a therapeutic agent as it is compatible to the human gut environment. Natural kidney stones were studied and the percentage of oxalate degradation by<i> Lac-tobacillus plantarum</i> was analysed in the minimal medium. Previous studies have reported the use of<i> Lactobacillu</i>s species as probiotic as well as for oxalate degradation from other sources like fermented food, human faeces. But this study aims to screen the potential <i>Lactobacillus plantarum</i> from dog faeces which could be used for oxalate degradation for the prophylaxis of hy-peroxaluria. This work represents a key milestone in the medical discoveries as an aid to human mankind as a whole.