Amplification of Herpes Simplex Virus Type 1 DNA in Human Geniculate Ganglia from Formalin-Fixed, Nonembedded Temporal Bones

Author:

Carreño Marcos1,OñA María2,Melón Santiago2,Llorente Jose Luis1,DÍAZ Juan José1,Suárez Carlos31

Affiliation:

1. Departments of Otolaryngology, Hospital Central de Asturias, Universidad de Oviedo, Spain.

2. Microbiology, Hospital Central de Asturias, Universidad de Oviedo, Spain.

3. Oviedo, Asturias, Spain

Abstract

Polymerase chain reaction (PCR) has provided new insights in molecular biology. Recently, some studies have been focused on temporal bone pathology, with amplification of DNA from fixed sections of celloidin-embedded bones. The purpose of our study was to elucidate the utility of PCR in detection of minor concentrations of DNA from nonoptimal stored samples. We obtained geniculate ganglia from 30 temporal bones preserved in formalin for a long time, without any process of embedding. By performing a nested PCR assay, we detected herpes simplex virus type 1 DNA in 13 of 30 ganglia (43%). We conclude therefore that study of temporal bones stored under poor conditions by PCR is possible, although there are some limitations when compared with fresh or optimally archived samples. (Otolaryngol Head Neck Surg 2000;123:508-11.)

Publisher

SAGE Publications

Subject

Otorhinolaryngology,Surgery

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