A High-Throughput Assay for Evaluation of Embryoid Bodies Using Local Redox Cycling-Based Electrochemical Chip Device

Abstract

We have previously proposed an excellent method to incorporate many electrochemical sensors into a single chip device using two sets of microelectrode array to induce local redox cycling, and we have designated the novel methodology as local redox cycling-based electrochemical (LR-EC) system. In this study, we developed a LRC-EC chip device consisting of 256 electrochemical sensors. At the electrochemical sensors, ring-type interdigitated array (IDA) electrodes were placed to induce local redox cycling. The LRC-EC chip device was applied to evaluate three-dimensional (3D) culture cells, such as embryoid bodies (EBs).