PRODUCTION, PURIFICATION AND IMMOBILIZATION OF TRIACYLGLYCEROL LIPASE FROM INDIGENOUS LIPOLYTIC MICROORGANISMS

Abstract

Triacylglycerol lipase was produced from locally isolated Aspergillus niger and Bacillus sp. using submerged fermentation. The process was optimized and lipase was purified and immobilized. Maximum lipase activity was obtained; with microorganisms cultured in medium containing 1% olive oil and 1% soybean meal as substrate, 72hrs incubation at 30°C, pH 6.0 and medium supplementation with 2% glucose, 1% peptone for A. niger, 72hrs incubation at 37°C, pH 8.0 and medium supplementation with 1% glucose, 1.5% peptone for Bacillus sp. The enzymes were purified using ammonium sulphate precipitation and ion exchange chromatography to achieve 13-14 fold purity with a yield ranging from 53-57%. Lewatite and Amberlite were found to be very good supports for the immoblization of bacterial and fungal lipases, respectively. The immobilization of lipase was proved to be an excellent tool for industrial applications of the enzyme.