Dynamics of non-specific humoral immunity factors in poultry which received feed supplement based on silkworm pupae

Abstract

The purpose of the study was to determine the focus and the intensity of the effect of the developed feed supplement based on the silkworm pupae on the nonspecific humoral immunity of poultry. The research was carried out on one-day-old broiler chickens, which were kept in standard vivarium conditions. The birds of first group were given the supplement in the morning, mixing with compound feed at the rate of 3.0 ml/kg of live weight. The second group was control. On the 20th, 27th, 34th and 48th days, 5 birds from each group were euthanized and blood was collected for clinical and biochemical studies. To determine the indicators of non-specific humoral immunity of birds after receiving feed supplement based on silkworm pupae in the blood serum of birds, the level of total protein, albumins, globulins, the concentration of circulating immune complexes of average molecular weight and seromucoids were determined by generally accepted methods. As a result of the use of a feed supplement based on silkworm pupae, there was determined an increase in the level of hemoglobin up to 16.0% and a slight activation of erythropoiesis, an increase in the level of humoral immunity, as evidenced by an increase in globulins up to 12.2% and circulating immune complexes up to 30.7%, and also inhibition of seromucoid synthesis by 15.4%. Thus, it has been concluded that the use of a feed supplement based on silkworm pupae allows to increase the level of non-specific humoral immunity of chickens, as evidenced by increased expression of its markers (globulins, circulating immune complexes), as well as inhibition of seromucoid synthesis. The revealed positive effect of the feed supplement based on silkworm pupae on the state of natural resistance can be considered as one of the factors of increasing the average daily weight gain of chickens by 39.0% in the first 7 days of life