Porcine reproductive and neonatal infections: Importance and threats of bacterial virophoria

Author:

Buzun A. I.1ORCID,Kolchyk O. V.1ORCID,Paliy A. P.1ORCID

Affiliation:

1. National Scientific Center “Institute of Experimental and Clinical Veterinary Medicine”, Kharkiv, Ukraine

Abstract

The provisions of the doctrine of transfer the epizootic process of dangerous viral infections to the enzootic process and their rooting in pig production through the integration of their pathogen into the pig microbiome in the form of comorbid viral and bacterial infections are substantiated. The aim of the study is to systematize the bacterial virophoria in the epizootology of porcine reproductive and neonatal infections (PRNI) as a component of the enzootic cycle of emergent infections in the pig industry of Ukraine. Classical swine fever (CSF) virus: attenuated strain ‘IECVM 03’; Aujeszky’s disease (AD) virus: epizootic strains of Ukrainian origin of AD virus ‘18v UNDIEV’; Teschen disease (TD) virus: epizootic strain ‘Bucha’. Epizootic strains: pasteurella bacteria, streptococcus, lacto- and bifidobacteria. According to the results of the study, it was found that the rotating magnetic field of the right direction promoted the adsorption of the CSF virus on pasteurella cells. The Aujeszky’s disease virus was adsorbed on the bacteria Salmonella choleraesuis No. 34, Bacterium bifidum and Lactobacillus casei with an efficiency of 15–45% in the pH range of 8.5–9.5, at neutral pH (7.4) no more than 1.5% of the virus was adsorbed, and at acidic pH (3.0) the AD virus was not adsorbed et all. On bacteria Pasteurella multocida No. 7, AD virus was adsorbed in the pH range of 8.5–9.5 with an efficiency of no more than 1.5%; at neutral pH (7.4), up to 50% of the virus was adsorbed, and at acidic pH (3.0), no more than 1.5% of AD virus was adsorbed. The interaction of TD virus with bifidobacteria inhibited viral reproduction in the body of infected polecats, but preserved the reproductive activity of teschovirus in the presence of streptococci. The rooting of dangerous viral infections (AD and TD, circovirus and parvovirus infections, reproductive and respiratory syndrome, and endemic porcine diarrhea) in pig production has always been accompanied by the ‘engraftment’ of their pathogens in the microbiome of pig production facilities in the form of comorbid (i. e. clinically manifested) and/or associated infections (i. e. similar to the group of Minimal Residual Human Diseases — Maladie Résiduelle Minimale, MRD). A key role in the establishment of these diseases and the formation of their stationary centers in pig production is played by the virophoria of bacteria synergistic with their pathogens, in particular as part of the etiologic microflora of reproductive and neonatal infections in pigs

Publisher

Kharkiv Entomological Society

Subject

General Medicine

Reference24 articles.

1. Buzun, A.-I. (2021) ‘L’microbiome de cobaye affecte-t-il au l’neurovirulence du Teshovirus envers l’porc? Analyse rétrospective des données’, Débats Scientifiques et Orientations Prospectives du Développement Scientifique: collection de papiers scientifiques ‘ΛΌГOΣ’ avec des matériaux de la II conférence scientifique et pratique internationale, Paris, 1 octobre 2021. Paris-Vinnytsia: La Fedeltà & Plateforme scientifique européenne, pp. 75–78. doi: 10.36074/logos-11.11.2022.14.

2. CE (The Council of Europe). (1986) European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes. (European Treaty Series, No. 123). Strasbourg: The Council of Europe. Available at: https://conventions.coe.int/treaty/en/treaties/html/123.htm.

3. CEC (The Council of the European Communities) (2010) ‘Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes’, The Official Journal of the European Communities, L 276, pp. 33–79. Available at: http://data.europa.eu/eli/dir/2010/63/oj.

4. Derevianko, S. V. (2019) ‘Antigenic and pathogenic properties of the Teschovirus A and Sapelovirus A strains, isolated from pigs and synanthropic animals in Ukraine’, [Antyhenni ta patohenni vlastyvosti shtamiv Teschovirus A ta Sapelovirus A, vydilenykh vid svynei i synantropnykh tvaryn v Ukraini], Microbiological Journal [Mikrobiolohichnyi Zhurnal], 81(6), pp. 83–96. doi: 10.15407/microbiolj81.06.083. [in Ukrainian].

5. Desenclos, J.-C. and De Valk, H. (2005) ‘Les maladies infectieuses émergentes : Importance en santé publique, aspects épidémiologiques, déterminants et prévention’, Médecine et Maladies Infectieuses, 35(2), pp. 49–61. doi: 10.1016/j.medmal.2004.09.005.

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