Study of biological properties of field isolates of cattle minor infections agents on homological cell cultures-Reference-Cited by-同舟云学术

Study of biological properties of field isolates of cattle minor infections agents on homological cell cultures

Published:2020-02-28 Issue:2 Volume:6 Page:16-19
ISSN:2411-0388
Container-title:Journal for Veterinary Medicine, Biotechnology and Biosafety
language:en
Short-container-title:J. Vet. Med. Biotechnol. Biosafety

Author:

Gorbatenko S. K.¹,Solodiankin O. S.¹^ORCID,Lymanska O. Yu.¹^ORCID,Rudova N. G.¹^ORCID,Kulyk I. O.¹,Kornieikov O. M.¹^ORCID,Stegniy M. Yu.¹^ORCID,Kuznetsova O. V.¹,Dunaeva O. V.²^ORCID,Gorbatenko V. P.³^ORCID

Affiliation:

1. National Scientific Center ‘Institute of Experimental and Clinical Veterinary Medicine’

2. Kharkiv National Medical University

3. Kharkiv State Zooveterinary Academy

Abstract

Biological properties of field isolates of bovine immunodeficiency virus and bovine foamy virus on homological cell cultures (fetal bovine lung and bovine coronary artery endothelial cells) were investigated. Pathogens of bovine slow infections, namely bovine immunodeficiency virus and bovine foamy virus, are able to integrate into cell cultures of homologous to cattle type, which is confirmed by the results of PCR. There has been determined the presence of genetic material of pathogens of bovine immunodeficiency (BIV) and spumavirus infection (BFV) in the cultivation of lymphocytes of field isolates in the culture of bovine coronary artery endothelial cells (BCAEC) at the level of 5th passage, and in the cell culture of fetal bovine lung (FBL) — at the level of 10th passage. In the process of integration of pathogens of immunodeficiency and spumavirus infection of cattle in continuous cell cultures FBL and BCAEC, morphological changes in the state of the monolayer by the principle of syncytiation and vacuolation are observed

Publisher

Kharkiv Entomological Society

Subject

Pharmacology (medical),Complementary and alternative medicine,Pharmaceutical Science

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