Glucose-Sensitive Biosensor Design by Zinc Ferrite (ZnFe2O4) Nanoparticle-Modified Poly (o-toluidine) Film-Reference-Cited by-同舟云学术

Glucose-Sensitive Biosensor Design by Zinc Ferrite (ZnFe2O4) Nanoparticle-Modified Poly (o-toluidine) Film

Published:2023-12-15 Issue:3 Volume:8 Page:202-213
ISSN:2458-8989
Container-title:Natural and Engineering Sciences
language:
Short-container-title:

Author:

OZYİLMAZ Ali Tuncay¹^ORCID,BAYRAM Esiye Irem¹^ORCID

Affiliation:

1. MUSTAFA KEMAL UNIVERSITY

Abstract

Glucose oxidase (GOD) immobilized poly(o-toluidine) (POT) coated Pt electrode was designed for glucose-sensitive biosensor. Since POT film structure affects enzyme activity, parameters of enzyme immobilization and POT synthesis conditions were optimized. Optimal monomer concentration for POT film synthesis was determined as 40 mM and the scanning rate was determined as 50 mV/s. As for the immobilization process results, GOD, glutaraldehyde (GAL) and chitosan (Chi) concentrations were decided as 2 mg/ml 0.10%, and 0.5% for the Pt/POT electrode. Zinc ferrite nanoparticle (ZnFe2O4NP) was immobilized together with POT film in the presence of GOD enzyme. It was revealed that ZnFe2O4NP increased the current responses and stability of the Pt/POT electrode.

Funder

Scientific Research Units of The University of Mustafa Kemal

Publisher

Iskenderun Technical University

Subject

General Medicine

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