A study on the primary materials for in vitro propagation of Petunia hybrida L.

Abstract

The aim of this study was to identify the primary materials for in vitro propagation of the Petunia hybrida L. The sterilisation method and the medium in different stages: shoot regeneration, rapid shoot multiplication, and the part of the in vitro plant used for rapid multiplication were identified. The study results showed that using nodal segments with axillary buds as the primary material and sterilising by HgCl2 0.1% in 10 minutes gave the best disinfection effect (survival rate reached 60%). Murashige and Skoog (MS) medium supplemented with 1 mg of BAP/l + 6.5 g agar/l + 30 g sacarose/l was reported as the best one for shoot regeneration (100% of shoot formed and number shoot per sample was 2.73), meanwhile, the mixture of MS + 1 mgBA/l + 0.2 mg IBA/l + 6.5 g agar/l + 30 g sacarose/l was considered as the best medium for rapid shoot multiplication (average shoots/sample reached 19.53). In vitro,leaf tissue is the most suitable vegetative organ for rapid multiplication, 100% of in vitro shoots regenerate through the form of callus, and the ratio of shoots/sample reached 21.53.