Effect of Basic Nutrient Media and Growth Regulatory Types on In vitro Propagation of Common Sage (Salvia officinalis)

Abstract

In this study, in vitro propagation of common sage (Salvia officinalis L.), which has high medicinal and commercial value, and the success of transferring in vitro propagated plants to external environment were investigated. The nodal parts of plants were used as explant source, and the effects of different nutrient media (MS and B5) on shoot growth in vitro and different doses (0, 0.5, 1.0, 2.0, 4.0 mg L-1)of various growth regulators (IAA; Indole acetic acid and IBA; Indole butyric acid) on root growth were investigated. MS medium had higher shoot number than B5 medium, and found to be more effective in obtaining shoots without vitrification, which is important for in vitro plant propagation. In vitro, the best rooting was observed in the medium containing 1.0 mg L-1 IBA, but in vitro rooted and unrooted shoots obtained from the medium containing 1.0 mg L-1 IAA showed higher acclimatization success than the others. All of the acclimatized plants were successfully transferred to the field conditions and their vitality continued. As a result, it offers an alternative propagation opportunity to determinate quickly reproduction of healthier plants from a single genotype of common sage for trading and breeding.