Author:
Miranda Luis Fernando Bandeira,Tabchoury Cinthia Pereira Machado,Cury Jaime Aparecido
Abstract
A validated protocol used to determine how much of total fluoride is bioavailable in toothpaste formulations was evaluated if it could be simplified regarding the following parameters: (1) concentration of the slurry (0.25–4%), (2) centrifugation speed (1,000–12,000 g) and time (1–10 min), and (3) incubation time for FPO32− ion hydrolysis and insoluble-F dissolution (15–60 min). Fresh and aged Na2FPO3/CaCO3 and fresh NaF/SiO2-based toothpastes were analyzed (n = 9). The improvements were the centrifugation at 1,000 g for 5 min is enough to separate insoluble-F and the time for FPO32− ion hydrolysis and insoluble-F dissolution can be shortened to 30 min.